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  Home > JCE Print > Journal of Chemical Education > Issues > 2002  > August  >
In the Laboratory
Application of Datalogger in Biosensing: A Glucose Biosensor
Martin M. F. Choi and Pui Shan Wong
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, SAR, PRC
Cover
August 2002
Vol. 79 No. 8
p. 982
Abstract
This article describes the use of a datalogger in conjunction with an oxygen sensor to fabricate a glucose biosensor. Glucose oxidase is first covalently immobilized on an eggshell membrane. The membrane is then used to cover the surface of an oxygen electrode. The detection scheme is based on the depletion of the dissolved oxygen upon exposure to glucose solution. The decrease in oxygen level is monitored and related to the glucose concentration. This biosensor has been successfully applied to determine the glucose content in beverage samples. These experiments provide suitable hands-on experience for undergraduate and postgraduate students to help them understand biosensors.
More Information
*  Citation
 Choi, Martin M. F.; Wong, Pui Shan. J. Chem. Educ. 2002 79 982.
*  Keywords
 Biochemistry; Carbohydrates; Datalogger*; Enzymes; Instrumental Methods; Laboratory Computing / Interfacing; Quantitative Analysis
*  History
 Created:
Last Updated:		 July 23, 2002
March 16, 2005
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