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  Home > JCE Print > Journal of Chemical Education > Issues > 2002  > January  >
In the Laboratory
The Binding Constant for Complexation of Bilirubin to Bovine Serum Albumin. An Experiment for the Biophysical Chemistry Laboratory
Kathryn R. Williams, Bhavin Adhyaru, and Russell E. Pierce
Department of Chemistry, University of Florida, Gainesville, FL 32611-7200

Stephen G. Schulman
Department of Medicinal Chemistry, University of Florida, Gainesville, FL 32610-0485

Cover
January 2002
Vol. 79 No. 1
p. 115
Abstract
Students use fluorescence spectroscopy to measure the binding constant for bilirubin-bovine serum albumin complexation by the Method of Continuous Variations. The experiment applies fundamental concepts of physical chemistry to a commercially available system of biological interest. The experiment is easily completed in a single laboratory period, and students obtain binding constants on the order of 10 7, in good agreement with literature values.
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Detailed background material, instructions for students, and an instructor's guide are available.
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*  Citation
 Williams, Kathryn R.; Adhyaru, Bhavin; Pierce, Russell E.; Schulman, Stephen G. J. Chem. Educ. 2002 79 115.
*  Keywords
 Biophysical Chemistry; Fluorescence Spectrometry; Laboratory Instruction; Proteins / Peptides; Thermodynamics
*  History
 Created:
Last Updated:		 December 3, 2001
March 16, 2005
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