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  Home > JCE Print > Journal of Chemical Education > Issues > 2002  > January  >
In the Classroom
Tested Demonstrations
Factors Affecting Reaction Kinetics of Glucose Oxidase
Kristin A. Johnson
Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706

checked by: Beth A. Kroa and Tony Yourey
Department of Chemisty, Kutztown University, Kutztown, PA 19530

Cover
January 2002
Vol. 79 No. 1
p. 74
Abstract
Basic principles of enzyme kinetics are demonstrated using the enzyme glucose oxidase. The glucose oxidase enzymatic reaction is coupled to horseradish peroxidase, which in turn catalyzes the oxidation of a dye to a bright blue-green color. The appearance of the blue-green dye is used to monitor the course of the reaction and is quite visible in a classroom setting. A series of reactions are arranged that vary the enzyme concentration, substrate concentration, temperature, and the substrate used in the reaction. By monitoring the rate of the color change in each beaker, the reaction kinetics of glucose oxidase in each series is observed.
More Information
*  Citation
 Johnson, Kristin A. J. Chem. Educ. 2002 79 74.
*  Keywords
 Biochemistry; Demonstrations; Enzymes; Kinetics; Proteins / Peptides
*  History
 Created:
Last Updated:		 December 3, 2001
April 21, 2005
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