|
|
| Home > JCE Print > Journal of Chemical Education > Issues >
2002
>
January
> |
|
In the Laboratory
|
|
|
|
Introducing Freshmen Students to the Practice of Solid-Phase Synthesis
|
Alpay Taralp, Can Hulusi Türkseven, Atilla Özgür Çakmak, and Ömer Çengel
Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul 81474, Turkey
|
|
January 2002
Vol. 79 No. 1
p. 87
|
|
|
|
|
|
|
| Abstract |
|
A one-semester laboratory project on solid-phase peptide chemistry was designed pedagogically to cater to freshman science students. The approach not only permitted multistep syntheses that would be considered impractical in solution, but also gave students insight into fundamental aspects of research at an early stage of development. Young scientists prepared Bz-Asn-Asn-Phe and Bz-Asn-Gln-Phe--peptides envisaged as potential competitive inhibitors of chymotrypsin. The synthesis, defined by an attachment-deprotection cycle, two elongation-deprotection cycles, and a benzoyl-capping protocol, was completed manually on Wang resin using Fmoc chemistry. Students quantified the yield of each condensation and deprotection reaction by measuring levels of dibenzylfulvene chromophore, a stoichiometrically afforded by-product. Benzoylation of the N-terminus was confirmed by employing a cadmium-ninhydrin reagent. The group also ascertained, through use of a chromogenic substrate, that chymotrypsin-catalyzed hydrolysis was impeded slightly when carried out in the presence of target peptides. Supplementary analyses supporting peptide purity and composition were given to students. Grading was based on laboratory participation, project proposals, reports, and a concluding slide-show presentation made to peers and colleagues. While the project was time-consuming overall, students acquired an impression of research work and an appreciation of the utility of solid-phase methods.
|
| Supplement |
Instructions for students and notes for demonstrators are available.
|
 Contents |
Folder JCE2002p0087W with Microsoft Word documents
|
| Download |
|
|
| More Information |
Citation |
Taralp, Alpay; Türkseven, Can Hulusi; Çakmak, Atilla Özgür; Çengel, Ömer. J. Chem. Educ. 2002 79 87.
|
Keywords |
Biotechnology; Collaborative / Cooperative Learning; Enzymes; Laboratory Instruction; Organic Synthesis; Proteins / Peptides; Undergraduate Research
|
History |
Created:
Last Updated: |
December 3, 2001
March 16, 2005
|
|
|
|
|
|
| Home > JCE Print > Journal of Chemical Education > Issues >
2002
>
January
> Page
87
|
|
|
| JCE HS CLIC |
|
Our Secondary School editors work hard to distill all the JCE materials to produce a fraction of particular interest to high school teachers. We call it CLIC.
|
| Contributions Welcome |
| JCE welcomes your submission |
| Advertisers |
| In recent years we have worked hard to better match our advertisers with our readers. When shopping for chemistry education materials, visit our advertisers' WWW sites first. |
| Be An Ambassador |
| Take JCE along on your outreach missions. Copies of the Journal, guest access to JCE Online, our publications catalog, and more are available for your participants. |
|
