JCE Online Journal of Chemical Education
 | Subscriptions  | Software Orders  | Support  | Contributors  | Advertisers  | 

JCE Print
   Current Issue
 Previous Issues
 Supplements
 Search JCE Index

JCE Digital Library
   DigiDemos
 QBank
 SymMath
 WebWare

JCE Software
   Latest Releases
 Software & Video
 Downloads
 Support

Only@JCE Online
   JCE Online Store
 JCE HS CLIC
 JCE Discussion Forums
   Biographical Snapshots
 ChemEd Resource Shelf
 Featured Molecules
 Hal's Picks
 Project Chemlab
 Reviewed WWW Sites
 "Web-Ed" Articles

About JCE
   Features
 Publications
 Operations
 Outreach
 Contact Us


  Home > JCE Print > Journal of Chemical Education > Issues > 2002  > March  >
In the Laboratory
Integrating HPLC and Electrochemistry: A LabVIEW-Based Pulsed Amperometric Detection System
Mark B. Jensen
Department of Chemistry, Concordia College, Moorhead, MN 56562
Cover
March 2002
Vol. 79 No. 3
p. 345
Abstract
A unique set of experiments coupling the fundamental principles of electrochemistry and liquid chromatography is described for the undergraduate instrumental analysis laboratory. Students begin by examining the voltammetric behavior of glucose in alkaline solution at a gold rotating-disk electrode (RDE). The results are applied to the development of an analytical method for the determination of glucose, fructose, and sucrose in fruit juices. These weakly acidic sugars are easily separated in alkaline solution using anion-exchange HPLC. The detection of these compounds, however, has presented some challenges. Their poor spectroscopic properties result in insensitive UV absorbance detection, and traditional constant-potential electrochemical detection is ineffective because products of the oxidation reactions remain adsorbed to the electrode surface, rendering it inactive. However, electrochemical detection is effective if the working electrode is subjected to a repeated series of potential pulses that alternately detects the analyte and cleans the electrode. Students use the glucose RDE data to construct this pulsed amperometric detection (PAD) waveform, and then use LabVIEW to both apply the waveform to the electrode and monitor the electrode current.
Supplement
Student handouts and notes for the instructor are available.
*  Contents JCE2002p0345W.doc (Microsoft Word)
*  Download
JCE2002p0345W.pdf

JCE2002p0345W.zip

JCE2002p0345W.sit
More Information
*  Citation
 Jensen, Mark B. J. Chem. Educ. 2002 79 345.
*  Keywords
 Analytical Chemistry; Chromatography; Electrochemistry; Instrumental Methods; Laboratory Instruction
*  History
 Created:
Last Updated:		 January 31, 2002
March 16, 2005
<< Previous Article Table of Contents Next Article >>
  Home > JCE Print > Journal of Chemical Education > Issues > 2002  > March  > Page 345


Subscriptions
 Subscription Info
 Order Forms

JCE HS CLIC

Our Secondary School editors work hard to distill all the JCE materials to produce a fraction of particular interest to high school teachers. We call it CLIC.
 JCE HS CLIC

Contributions Welcome
JCE welcomes your submission
 Contributors' Corner

Advertisers
In recent years we have worked hard to better match our advertisers with our readers. When shopping for chemistry education materials, visit our advertisers' WWW sites first.
 Recent Advertisers

Be An Ambassador
Take JCE along on your outreach missions. Copies of the Journal, guest access to JCE Online, our publications catalog, and more are available for your participants.
 Outreach with JCE

Comments to
jceonline@chem.wisc.edu		 Copyright © Division of Chemical Education, Inc., American Chemical Society.
All rights reserved.