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  Home > JCE Print > Journal of Chemical Education > Issues > 2002  > March  >
In the Laboratory
Using Infrared Spectroscopy to Investigate Protein Structure
Janet Olchowicz, Deidra R. Coles, Lori E. Kain, and Gina MacDonald
Department of Chemistry, James Madison University, Harrisonburg, VA 22807
Cover
March 2002
Vol. 79 No. 3
p. 369
Abstract
This report describes a laboratory that employs infrared spectroscopy to observe protein secondary structure. The techniques utilized are suitable for all levels of biochemistry or biophysical chemistry laboratories. Students first obtain absorbance spectra on proteins that are predominately composed of either a helixes or b sheets in order to understand how infrared is used to study secondary structure. For an extended laboratory we describe how infrared spectroscopy can be used to monitor the unfolding of myoglobin. The same techniques could easily be applied to any protein or to monitor the unfolding of any predominately a- helical protein whose characterization is of interest.
Supplement
Instructor's notes and a student handout for this experiment are available.
*  Contents JCE2002p0369W1.doc and JCE2002p0369W2.doc (Microsoft Word)
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More Information
*  Citation
 Olchowicz, Janet; Coles, Deidra R.; Kain, Lori E.; MacDonald, Gina. J. Chem. Educ. 2002 79 369.
*  Keywords
 Biochemistry; Biophysical Chemistry; IR Spectroscopy; Laboratory Instruction; Proteins / Peptides
*  History
 Created:
Last Updated:		 January 31, 2002
March 16, 2005
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