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  Home > JCE Print > Journal of Chemical Education > Issues > 2002  > March  >
In the Laboratory
The Cis-Trans Equilibrium of N-Acetyl-L-Proline. An Experiment for the Biophysical Chemistry Laboratory
Kathryn R. Williams, Bhavin Adhyaru, Igor German, and Eric Alvarez
Department of Chemistry, University of Florida, Gainesville, FL 32611-7200
Cover
March 2002
Vol. 79 No. 3
p. 372
Abstract
Students in the biophysical chemistry laboratory use 1H NMR to evaluate the equilibrium constant for the cis-trans isomerism of N-acetyl-L-proline. The four solvent systems (45:55 d6-benzene-CDCl3, d6-acetone, D2O with pH < 2, and D2O with pH > 7) demonstrate the effects of solvent polarity and aqueous pH.
Supplement
Background material and instructions for students and guidelines for the instructor are available.
*  Contents JCE2002p0372W.doc (Microsoft Word)
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More Information
*  Citation
 Williams, Kathryn R.; Adhyaru, Bhavin; German, Igor; Alvarez, Eric. J. Chem. Educ. 2002 79 372.
*  Keywords
 Amino Acids; Biophysical Chemistry; Laboratory Instruction; NMR Spectrometry; Proteins / Peptides
*  History
 Created:
Last Updated:		 January 31, 2002
March 16, 2005
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