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  Home > JCE Print > Journal of Chemical Education > Issues > 2003  > April  >
In the Laboratory
Analysis of Carbon Monoxide in Blood
Benjamin P. Huddle and Joseph C. Stephens
Department of Chemistry, Roanoke College, Salem, VA 24153
Cover
April 2003
Vol. 80 No. 4
p. 441
Abstract
Forensic tests used to perform the qualitative and quantitative analyses of carbon monoxide in blood are described. The qualitative test uses the diffusion of CO, which is released from blood by reaction with H2SO4, into a PdCl2 solution in a Conway cell and the resultant formation of a palladium mirror. The quantitative analysis is based on the absorption of visible light by carboxyhemoglobin at 541 nm and reduced hemoglobin at 555 nm. Both procedures are suitable for undergraduate chemistry experiments.
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Detailed laboratory instructions and notes for the instructor are available.
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*  Citation
 Huddle, Benjamin P.; Stephens, Joseph C. J. Chem. Educ. 2003 80 441.
*  Keywords
 Forensic Chemistry; Gases; Laboratory Instruction; Toxicology; UV-Vis Spectroscopy
*  History
 Created:
Last Updated:		 March 10, 2003
February 28, 2005
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