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  Home > JCE Print > Journal of Chemical Education > Issues > 2003  > December  >
In the Laboratory
Simple Experiments To Demonstrate Proton Flux in Pseudomonas after Alkaline or Acidic Stress
Gabriela Previtali, Walter Giordano, and Carlos E. Domenech
Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, Córdoba, Argentina
Cover
December 2003
Vol. 80 No. 12
p. 1468
Abstract
This laboratory introduces chemistry students to the ability of microorganisms to adapt to acidic or alkaline environmental conditions. A laboratory experiment to ascertain the bacterial response to the stress produced by suspension in different pH solutions has been developed. The experiment may be performed in several versions depending on the availability of lab equipment and the chemistry level of the students. This laboratory experiment has the pedagogical advantage of giving chemistry students experience with the application of various pH levels to a biological system and enables the students to expand their understanding of pH to mean more than a strictly chemical concept.
Supplement
Instructions for the students and notes for the instructor are available.
*  Contents JCE2003p1468W.doc (Microsoft Word)
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*  Citation
 Previtali, Gabriela; Giordano, Walter; Domenech, Carlos E. J. Chem. Educ. 2003 80 1468.
*  Keywords
 Acid-Base Chemistry; Biochemistry; Laboratory Instruction
*  History
 Created:
Last Updated:		 October 31, 2003
February 28, 2005
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