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Information, Textbooks, Media, Resources
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Computer Bulletin Board
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Chemical Applications of a Programmable Image Acquisition System
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Paul J. Ogren, Ian Henry, Steven E. S. Fletcher, and Ian Kelly
Department of Chemistry, Earlham College, Richmond, IN 47374
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June 2003
Vol. 80 No. 6
p. 699
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| Abstract |
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Image analysis is widely used in chemistry, both for rapid qualitative evaluations using techniques such as thin layer chromatography (TLC) and for quantitative purposes such as well-plate measurements of analyte concentrations or fragment-size determinations in gel electrophoresis. This paper describes a programmable system for image acquisition and processing that is currently used in the laboratories of our organic and physical chemistry courses. It has also been used in student research projects in analytical chemistry and biochemistry. The potential range of applications is illustrated by brief presentations of four examples: (1) using well-plate optical transmission data to construct a standard concentration–absorbance curve; (2) the quantitative analysis of acetaminophen in Tylenol and acetylsalicylic acid in aspirin using TLC with fluorescence detection; (3) the analysis of electrophoresis gels to determine DNA fragment sizes and amounts; and, (4) using color change to follow reaction kinetics. The supplemental material in JCE Online contains information on two additional examples: deconvolution of overlapping bands in protein gel electrophoresis, and the recovery of data from published images or graphs. The JCE Online material also presents additional information on each example, on the system hardware and software, and on the data analysis methodology.
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| Supplement |
Further information on the examples, the system components, the background planar fitting procedure, color versions of some of the figures, and more detailed information on the LabVIEW and spreadsheet computations are available in this issue of JCE Online. Two additional examples are also presented: (a) the electrophoresis of a protein mixture, with deconvolution of overlapping peaks, and (b) use of the software to capture data from published images and graphs.
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JCE2003p0699W.doc (Microsoft Word)
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| Download |
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| More Information |
Citation |
Ogren, Paul J.; Henry, Ian; Fletcher, Steven E. S.; Kelly, Ian. J. Chem. Educ. 2003 80 699.
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Keywords |
Analytical Chemistry; Biochemistry; Instrumental Methods; Laboratory Computing / Interfacing; Laboratory Equipment / Apparatus; Organic Chemistry; Physical Chemistry
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History |
Created:
Last Updated: |
May 5, 2003
February 28, 2005
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