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  Home > JCE Print > Journal of Chemical Education > Issues > 2003  > March  >
In the Laboratory
Topics in Chemical Instrumentation
Microscale Capillary Electrophoresis: A Complete Instrumentation Experiment for Chemistry Students at the Undergraduate Junior or Senior Level
Ruben T. Almaraz
Biomedical Engineering Program, California State University, Sacramento, CA 95819

Maria Kochis
CSU Library Reference Department, California State University, Sacramento, CA 95819

Cover
March 2003
Vol. 80 No. 3
p. 316
Abstract
A microscale capillary electrophoresis (CE) system was created with conventional devices. Mechanical and thermal techniques were implemented in the creation of a microchip in a microscope glass slide for analyte separation. The detection system consisted of a high-intensity LED used as light source and a photodiode, with an amplifier integrated in the same chip, as the detector. LabVIEW was used for analysis and acquisition of data from the system in which two commercial dyes, Evans Blue and Night Blue, were separated. The performance of the microsystem was compared to a conventional CE instrument. It was found that although the microscale CE has a relatively low sensitivity, it compensates by providing better resolution and shorter time for analysis and low cost.
Supplement
Instructions for the students are available.
*  Contents JCE2003p0316W.doc (Microsoft Word)
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JCE2003p0316W.zip

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More Information
*  Citation
 Almaraz, Ruben T.; Kochis, Maria. J. Chem. Educ. 2003 80 316.
*  Keywords
 Electrophoresis; Instrumental Methods; Laboratory Instruction; Microscale
*  History
 Created:
Last Updated:		 February 6, 2003
February 28, 2005
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