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  Home > JCE Print > Journal of Chemical Education > Issues > 2003  > May  >
In the Laboratory
Milk as a Drug Analysis Medium: HPLC Determination of Isoniazid
Enzo Sottofattori, Riccardo Raggio, and Olga Bruno
Dipartimento di Scienze Farmaceutiche, Universitá degli Studi di Genova, 3-16132 Genova, Italy
Cover
May 2003
Vol. 80 No. 5
p. 547
Abstract
The use of milk as a medium for drug analysis in a graduate-laboratory experiment is proposed. This project, designed with an interdisciplinary approach, gives the students the opportunity to safely manipulate biological fluids and to learn their basics. The experiment is modeled on a real-life analytical problem, aimed at determining the isoniazid content in milk using simple HPLC equipment. The methodology requires the derivatization of isoniazid with cinnamaldehyde to form a hydrazone suitable for UV detection. The present method could easily be adapted to other drug analysis. Details of the experimental procedure are described along with representative student results. This experiment provides experience in quantitative chromatographic techniques and in the chemical treatment of biological fluids.
Supplement
Instructions for the students and notes for the instructor are available.
*  Contents
*  Download
JCE2003p0547W.pdf
More Information
*  Citation
 Sottofattori, Enzo; Raggio, Riccardo; Bruno, Olga. J. Chem. Educ. 2003 80 547.
*  Keywords
 Chromatography; Drugs / Pharmaceuticals; Food Science; Proteins / Peptides
*  History
 Created:
Last Updated:		 March 28, 2003
February 28, 2005
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