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  Home > JCE Print > Journal of Chemical Education > Issues > 2003  > November  >
In the Laboratory
Investigation of Model Cell Membranes with Raman Spectroscopy: A Biochemistry Laboratory Experiment
Norman C. Craig, William H. Fuchsman, and Nanette N. Lacuesta
Department of Chemistry, Oberlin College, Oberlin, OH 44074-1083
Cover
November 2003
Vol. 80 No. 11
p. 1282
Abstract
New developments that extend the usefulness of Raman spectroscopy for the investigation of biological systems are described. One of these methods, excitation with a near-infrared laser at 1064 nm and detection with a Fourier transform infrared spectrometer, is applied to the investigation of temperature profiles of dispersions of phospholipids in water. These dispersions are models of biomembranes. This experiment for the undergraduate biochemisty laboratory gives students experience applying vibrational spectroscopy to the investigation of the molecular properties of biomembranes. The basis of the Raman effect is reviewed.
Supplement
The laboratory writeup of the experiment, including details of the experimental methods, specifics of apparatus used in sample preparation and of the construction of the variable temperature sample holder are available.
*  Contents JCE2003p1282W.doc (Microsoft Word)
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More Information
*  Citation
 Craig, Norman C.; Fuchsman, William H.; Lacuesta, Nanette N. J. Chem. Educ. 2003 80 1282.
*  Keywords
 Biochemistry; Fourier Transform Techniques; Laboratory Instruction; Lasers / Laser Spectroscopy; Lipids; Raman Spectroscopy
*  History
 Created:
Last Updated:		 October 2, 2003
February 28, 2005
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