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  Home > JCE Print > Journal of Chemical Education > Issues > 2004  > April  >
In the Laboratory
Topics in Chemical Instrumentation
Fluorescence Microscopy of Single Molecules
Jan Zimmermann, Arthur van Dorp, and Alois Renn
Physical Chemistry Laboratory, Swiss Federal Institute of Technology, ETH Hoenggerberg, CH 8093 Zürich, Switzerland
Cover
April 2004
Vol. 81 No. 4
p. 553
Abstract
A wide-field microscopy setup suitable for single-molecule detection at ambient temperature is described. The instrument, a lab-built inverted microscope that is operated in epi-fluorescence mode, allows the observation of fluorescing single molecules in real time. The instrument is used in an advanced laboratory course to investigate the influence of oxygen on molecular fluorescence at the single-molecule level. In this context students can be experimentally introduced into the world of single quantum systems.
More Information
*  Citation
 Zimmermann, Jan ; van Dorp, Arthur; Renn, Alois. J. Chem. Educ. 2004 81 553.
*  Keywords
 Dyes / Pigments; Excited States / Energy Transfer; Fluorescence Spectrometry; Luminescence; Nanotechnology; Photochemistry; Physical Chemistry
*  History
 Created:
Last Updated:		 March 3, 2004
February 23, 2005
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