In response to the increasing importance of biochemical applications in modern analytical chemistry and the high level of interest in biochemistry among students at Cornell College, we sought to incorporate more biochemical concepts and methods into the sophomore-level analytical course. This report describes bioanalyical experiments that have been developed or adapted using an HPLC and a UV–visible spectrophotometer. Specific experiments include amino acid analysis, biochromatography, and total protein analysis. These improvements have the potential to increase our students' understanding of selected biochemistry topics and enhance their ability to apply an analytical approach to solving biochemical problems.
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Citation
Strong, Cynthia; Ruttencutter, Jeffrey. J. Chem. Educ.2004 81 1706.
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