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2004
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February
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In the Laboratory
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Fluorescence, Absorption, and Excitation Spectra of Polycyclic Aromatic Hydrocarbons as a Tool for Quantitative Analysis
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A. M. Rivera-Figueroa, K. A. Ramazan, and B. J. Finlayson-Pitts
Department of Chemistry, University of California, Irvine, CA 92697-2025
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February 2004
Vol. 81 No. 2
p. 242
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| Abstract |
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An experiment suitable for college junior or senior students in the analytical instrumental analysis laboratory that demonstrates the analysis of PAHs (benzo[a]anthracene, benzo[k]fluoranthene, benzo[a]pyrene, chrysene, and phenanthrene) using absorption and fluorescence spectroscopy is described. This experiment is carried out during one seven-hour instrumental analysis laboratory. It could also be used in a physical chemistry laboratory to demonstrate fundamental spectroscopic and photochemical principles. A Beer–Lambert plot for an absorption peak of each PAH was obtained and used to determine the molar absorptivities. The effect of heavy atoms as quenchers of fluorescence was studied by using 1-bromoheptane and 1,7-dibromoheptane, and Stern–Volmer plots were prepared to determine the ratios of the quenching rate constants to the fluorescence rate constant, kQ/kf. The experiment is also useful as an experiment preceding the determination of PAHs by HPLC with absorption and fluorescence detection as described earlier (J. Chem. Educ. 1998, 75, 1599).
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| Supplement |
Instructions for the students and notes for the instructor are available.
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 Contents |
JCE2004p0242W.doc (Microsoft Word)
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| Download |
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| More Information |
Citation |
Rivera-Figueroa, A. M.; Ramazan, K. A.; Finlayson-Pitts, B. J. J. Chem. Educ. 2004 81 242.
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Keywords |
Analytical Chemistry; Environmental Chemistry; Fluorescence Spectrometry; Laboratory Instruction; Molecular Properties / Structure; Physical Chemistry; Quantitative Analysis; UV-Vis Spectroscopy
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History |
Created:
Last Updated: |
January 5, 2004
February 25, 2005
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Link to Featured Molecules added (April 2004).
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| Home > JCE Print > Journal of Chemical Education > Issues >
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February
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242
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