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  Home > JCE Print > Journal of Chemical Education > Issues > 2004  > March  >
In the Laboratory
Investigation of Cu(II) Binding to Bovine Serum Albumin by Potentiometry with an Ion Selective Electrode: A Biophysical Chemistry Experiment for the Undergraduate Curriculum
Jie Liu
Department of Chemistry, Nantong Medical College, Nantong, Jiangsu 226001, china
Cover
March 2004
Vol. 81 No. 3
p. 395
Abstract
The binding of ligands to biological macromolecules should be included in the laboratory of the undergraduate biophysical chemistry curriculum. An interesting experiment investigating Cu(II) ion binding to bovine serum albumin (BSA) by using potentiometry with the ion selective electrode is described and offers attractive features over some traditional methodologies. Students analyze experimental data to obtain the average number of Cu(II) binding sites on a BSA molecule and the values of corresponding binding constants, either by a specifically designed computer program for regression to the binding equation, or by a graphic method in terms of Scatchard model.
Supplement
An expanded version of experimental procedures for students and notes for instructors are available.
*  Contents JCE2004p0395W.doc (Microsoft Word)
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More Information
*  Citation
 Liu, Jie. J. Chem. Educ. 2004 81 395.
*  Keywords
 Biochemistry; Biophysical Chemistry; Copper; Electrochemistry; Laboratory Instruction; Proteins / Peptides
*  History
 Created:
Last Updated:		 February 3, 2004
February 18, 2005
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