A Computer-Based Undergraduate Exercise Using Internet-Accessible Simulation Software for the Study of Retention Behavior and Optimization of Separation Conditions in Ion Chromatography
Paul R. Haddad, Matthew J. Shaw, John E. Madden, and Greg W. Dicinoski
Australian Centre for Research on Separation Science, School of Chemistry, University of Tasmania, Hobart 7001, Tasmania, Australia
An ion chromatography exercise is described that uses a free Internet-based software package, Virtual Column 2, to simulate retention behavior and to optimize separation conditions. Virtual Column 2 permits the detailed study of retention behavior of inorganic anions on a Dionex AS11 column using isocratic hydroxide eluents and also on a Dionex AS4A–SC column using carbonate-bicarbonate eluents. Collection of retention data from Virtual Column 2 allows students to investigate the effects of variation in eluent composition on retention, to examine separation selectivity in ion chromatography, and to evaluate the performance of simple retention models. Because the retention data provided by the software are based on actual experimental measurements an accurate evaluation of these models is possible. Effects of peak shape on resolution are also studied and the software can be used to perform computer-assisted optimization of separation conditions. The entire exercise, including familiarization with the software, takes about four hours and can be adjusted to fit into a shorter time frame if necessary.
Supplement
Questions that the instructor might ask the students after completing the exercise are available.
Contents
JCE2004p1293W.doc (Microsoft Word)
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Citation
Haddad, Paul R.; Shaw, Matthew J.; Madden, John E.; Dicinoski, Greg W. J. Chem. Educ.2004 81 1293.
Keywords
Analytical Chemistry; Computer Assisted Instruction; Instrumental Methods; Ion Exchange; Separation Science; Water / Water Chemistry
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