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  Home > JCE Print > Journal of Chemical Education > Issues > 2005  > August  >
In the Laboratory
Cytochrome c: A Biochemistry Laboratory Course
John B. Vincent and Stephen A. Woski
Department of Chemistry and Coalition for Biomolecular Products, The University of Alabama, Tuscaloosa, AL 35487-0336
Cover
August 2005
Vol. 82 No. 8
p. 1211
Abstract
A unified biochemistry laboratory course has been developed. Rather than using miscellaneous laboratory experiments, the concept behind this course is to focus on a theme: the process of biochemical research. The laboratory deals entirely with one protein, cytochrome c, simulating the experience of a real-world research project. The laboratory is built around team-learning: groups of students isolate cytochrome c, characterize it by a variety of biochemical and bioinorganic methods, attempt to determine its function, and finally alter the protein to produce a modifier of oxidative nucleic acid damage. In this way, the students are introduced in general terms to the techniques necessary to research a new biochemical problem. Overall, by exposing students to the excitement of research problems, it is hoped their interests in pursuing careers in chemical or biochemical research will be stimulated.
More Information
*  Citation
 Vincent, John B.; Woski, Stephen A. J. Chem. Educ. 2005 82 1211.
*  Keywords
 Biochemistry; Bioinorganic Chemistry; Chromatography; Curriculum; Electrophoresis; Enzymes; EPR / ESR Spectroscopy; Inquiry-Based / Discovery Learning; NMR Spectroscopy; Nucleic Acids / DNA / RNA; Proteins / Peptides; Upper-Division Undergraduate; UV-Vis Spectroscopy
*  History
 Created:
Last Updated:		 July 5, 2005
July 8, 2005
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