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  Home > JCE Print > Journal of Chemical Education > Issues > 2005  > December  >
In the Laboratory
Topics in Chemical Instrumentation
Sequential-Injection Analysis: Principles, Instrument Construction, and Demonstration by a Simple Experiment
A. Economou, P. D. Tzanavaras, and D. G. Themelis
Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki 541 24, Greece
Cover
December 2005
Vol. 82 No. 12
p. 1820
Abstract
This article is a brief introduction to sequential-injection analysis (SIA), a novel automated analytical technique devised to replace flow-injection analysis (FIA). The principles and requirements of SIA are explained and the different components of a generic SIA apparatus are described. The construction of a simple SIA analyzer from commercially available components is reported together with the control and acquisition software developed in LabVIEW. The typical chemical and instrumental parameters to be considered in a typical SIA experiment and the analytical utility of the technique are demonstrated by using the photometric determination of phosphates in urine by means of the "molybdenum blue" reaction. This experiment is intended to form part of the laboratory coursework in the course of Instrumental Chemical Analysis.
Supplement
Instructions for the students and notes for the instructor are available.
*  Contents JCE2005p1820W.doc (Microsoft Word)
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*  Citation
 Economou, A.; Tzanavaras, P. D.; Themelis, D. G. J. Chem. Educ. 2005 82 1820.
*  Keywords
 Analytical Chemistry; Hands-On Learning / Manipulatives; Instrumental Methods; Laboratory Computing / Interfacing; Laboratory Instruction; Quantitative Analysis; Upper-Division Undergraduate
*  History
 Created:
Last Updated:		 October 25, 2005
November 4, 2005
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