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2005
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July
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In the Laboratory
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Kinetics of Alcohol Dehydrogenase-Catalyzed Oxidation of Ethanol Followed by Visible Spectroscopy
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Kestutis Bendinskas
Department of Chemistry, SUNY at Oswego, Oswego, NY 13126
Christopher DiJiacomo, Allison Krill, and Ed Vitz
Department of Physical Sciences, Kutztown University, Kutztown, PA 19539
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July 2005
Vol. 82 No. 7
p. 1068
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| Abstract |
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A two-week biochemistry experiment was introduced in the second-semester general
chemistry laboratory to study the oxidation of ethanol in vitro in the presence
of the enzyme alcohol dehydrogenase (ADH). This reaction should pique student
interest because the same reaction also occurs in human bodies when alcoholic
drinks are consumed. Procedures were developed to follow the biochemical reaction
by visible spectroscopy and to avoid specialized equipment. The effect of substrate
concentration on the rate of this enzymatic reaction was investigated during
the first week. One of the solutions was allowed to reoxidize in air and recover
the color in absence of additional ethanol to demonstrate the fact that open
living systems tend to reach equilibrium with their oxidizing surroundings
in the absence of an influx of energy in form of reducing agents. The effects
of temperature, pH, the specificity of the enzyme to several substrates, and
the enzyme's inhibition by heavy metals were explored during the second week.
An anonymous voluntary survey expressed student satisfaction with the lab.
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| Supplement |
Student handouts, note for the instructor, reagent preparation directions, detailed
safety information, MS Excel templates for data collection and calculations,
survey materials, Vernier LoggerPro files, WebCT student pre-laboratory questions
in both native and MSWord format, and answers to pre-laboratory and post-laboratory
questions are available.
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 Contents |
Folder JCE2005p1068W containing: Answers.doc, Evaluation.doc, Instructor_notes.doc,
Student_manual.doc, WebCT_Q&A.doc (Microsoft Word); SpreadsheetWk1.xls, SpreadsheetWk2.xls
(Microsoft Excel); WebCT_week1.txt, and WebCT_week2.txt (WebCT).
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| Download |
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| More Information |
Citation |
Bendinskas, Kestutis; DiJiacomo, Christopher; Krill, Allison; Vitz, Ed. J. Chem. Educ. 2005 82 1068.
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Keywords |
Alcohols; Biochemistry; Biophysical Chemistry; Enzymes; First-Year Undergraduate / General; Food Science; Hands-On Learning / Manipulatives; Kinetics; Laboratory Instruction; Oxidation / Reduction; Reactions; UV-Vis Spectroscopy
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History |
Created:
Last Updated: |
May 31, 2005
June 8, 2005
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| Home > JCE Print > Journal of Chemical Education > Issues >
2005
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July
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1068
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