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  Home > JCE Print > Journal of Chemical Education > Issues > 2005  > June  >
In the Laboratory
A LED Microtiter Plate Reader
James Gordon and Sandra Tye
Division of Science and Mathematics, Central Methodist University, Fayette, MO 65248
Cover
June 2005
Vol. 82 No. 6
p. 903
Abstract
An inexpensive photometric detector system was designed for use with a 96-well microtiter plate. The absorbance of solutions in the wells was measured by using a light-emitting diode as the light source and a Vernier LabPro data-collection interface with a light sensor as the detector. The feasibility of the system was demonstrated with a linear absorbance versus concentration calibration curve constructed from solutions of yellow food coloring. The results of an ELISA experiment for the detection of IgG proteins in rabbit serum confirmed the usefulness of the device for such experiments.
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*  Contents JCE2005p0903W.doc (Microsoft Word)
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*  Citation
 Gordon, James; Tye, Sandra. J. Chem. Educ. 2005 82 903.
*  Keywords
 Biochemistry; Biophysical Chemistry; Calculator-Based Learning; Dyes / Pigments; First-Year Undergraduate / General; Hands-On Learning / Manipulatives; Laboratory Equipment / Apparatus; Laboratory Instruction; Quantitative Analysis; Upper-Division Undergraduate; UV-Vis Spectroscopy
*  History
 Created:
Last Updated:		 April 27, 2005
May 11, 2005
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