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  Home > JCE Print > Journal of Chemical Education > Issues > 2006  > April  >
In the Laboratory
An Enzyme Kinetics Experiment Using Laccase for General Chemistry
Yaqi Lin and Patrick M. Lloyd
Department of Physical Sciences, Kingsborough Community College, City University of New York, Brooklyn, NY 11235
Cover
April 2006
Vol. 83 No. 4
p. 638
Abstract
This article describes the use of laccase, an oxidoreductase enzyme, to study the effects of enzyme catalysts on reaction rates. The experiment is intended for students in the second semester of the general chemistry sequence. The effects of environmental conditions on enzyme activity are studied by varying pH and by adding an inhibitor of laccase. The effect of substrate structure on enzyme activity is also tested by comparing laccase activity in catechol and syringaldazine solutions. Students perform the experiment using a spectrophotometer and a timer. Enzyme activities are then calculated and compared from the slopes of the time-dependent absorbance changes.
Supplement
Instructions for the students and notes for the instructor are available.
*  Contents JCE2006p0638W.doc (Microsoft Word)
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JCE2006p0638W.pdf

JCE2006p0638W.zip
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*  Citation
 Lin, Yaqi; Lloyd, Patrick M. J. Chem. Educ. 2006 83 638.
*  Keywords
 Aldehydes / Ketones; Analytical Chemistry; Bioanalytical Chemistry; Biochemistry; Catalysis; Enzymes; First-Year Undergraduate / General; Hands-On Learning / Manipulatives; Kinetics; Laboratory Instruction; UV-Vis Spectroscopy
*  History
 Created:
Last Updated:		 2/24/2006
3/6/2006
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