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  Home > JCE Print > Journal of Chemical Education > Issues > 2006  > August  >
In the Laboratory
Label-Free Detection of DNA Hybridization by Cyclic Voltammetry. An Advanced Undergraduate Analytical Chemistry Laboratory Experiment
Temitope Aiyejorun, Janusz Kowalik, Jiri Janata, and Mira Josowicz
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400
Cover
August 2006
Vol. 83 No. 8
p. 1208
Abstract
A label-free electrochemical detection method for DNA hybridization has been developed and tested in an advanced analytical chemistry course. A set of experiments was designed to explore several concepts relevant to electropolymerization of intrinsically conducting polymers, their use as an electrically controlled ion-exchanger of chloride ions, and their ability to bind oligonucleotides through a noncovalent interaction with magnesium ions used as a linker between the polymer and the DNA strand. The electrostatic barrier on the modified electrode created by the phosphate groups of the probe DNA attached to the magnesium ion is modified by DNA hybridization. Changes in the transfer rate of chloride ions of the "label-free DNA detector" are detected as variations in cyclic voltammograms. The basic approach for the detection of the DNA hybridization event relies on the subtraction of cyclic voltammograms recorded before and after DNA hybridization. Different aspects of the experiments can be emphasized, depending on the interests of students and the instructor.
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Detailed experimental procedures, student handout, instructor notes, and spectra are available.
*  Contents JCE2006p1208W.doc (Microsoft Word)
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*  Citation
 Aiyejorun, Temitope; Kowalik, Janusz; Janata, Jiri; Josowicz, Mira. J. Chem. Educ. 2006 83 1208.
*  Keywords
 Analytical Chemistry; Biophysical Chemistry; Electrochemistry; Hands-On Learning / Manipulatives; Kinetics; Laboratory Instruction; Nucleic Acids / DNA / RNA; Undergraduate Research; Upper-Division Undergraduate
*  History
 Created:
Last Updated:		 6/21/2006
6/30/2006
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