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In the Laboratory
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Investigation of Fluorescence Lifetime Quenching of Ru(bpy)32+ by Oxygen Using a Pulsed Light-Emitting Diode
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David A. Rusak, William H. James, III, Maria J. Ferzola, and Michael J. Stefanski
Department of Chemistry, University of Scranton, Scranton, PA 18510-4626
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December 2006
Vol. 83 No. 12
p. 1857
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| Abstract |
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The measurement of fluorescence lifetimes in an undergraduate laboratory typically requires access to pulsed lasers. Commonly, a N2 laser is used to pump a dye or a frequency doubled Nd:YAG is used at 532 nm. While it is certainly valuable to introduce undergraduates to these lasers, it is also possible to perform fluorescence lifetime experiments using a pulsed-LED as an excitation source. The use of pulsed-LEDs does away with the safety concerns associated with lasers and laser dyes and simplifies the experimental procedure. In our experiment, the fluorescence lifetime of Ru(bpy)32+ is measured as a function of oxygen concentration. The concentration of oxygen is varied by addition of sodium sulfite to the aqueous solution and is monitored. Stern–Volmer plots are constructed, and the observed quenching rate constant is compared to the calculated bimolecular rate constant. Students also become acquainted with the simple circuit used to pulse the LED and with the use of the oscilloscope.
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| Supplement |
Instructions for the students, including sections on theory, the circuit, the instrument, and procedure, are available
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| More Information |
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Citation |
Rusak, David A.; James, William H., III; Ferzola, Maria J.; Stefanski, Michael J. J. Chem. Educ. 2006 83 1857.
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Keywords |
Analytical Chemistry; Coordination Compounds; Fluorescence Spectroscopy; Hands-On Learning / Manipulatives; Instrumental Methods; Laboratory Equipment / Apparatus; Laboratory Instruction; Physical Chemistry; Upper-Division Undergraduate
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History |
Created:
Last Updated: |
10/30/2006
11/7/2006
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 Caution! | | Experiments, laboratory exercises, lecture demonstrations, and other descriptions of the use of chemicals, apparatus, instruments, computers, and computer interfaces are presented in the Journal of Chemical Education as illustrative of new or improved ideas or concepts in chemistry instruction and are directed at qualified teachers. Although every effort is made to assure and encourage safe practices and safe use of chemicals, the Journal of Chemical Education cannot assume responsibility for uses made of its published materials. Many chemicals are hazardous. Precautions for the safe use of hazardous chemicals and directions for their proper disposal are described in the Material Safety Data Sheets and on the labels. We strongly urge all those planning to use materials from our pages to make choices and to develop procedures for laboratory and classroom safety in accordance with local needs and situations. |
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