JCE Online Journal of Chemical EducationDivision of Chemical Education, American Chemical SocietyAmerican Chemical Society
 | Subscriptions  | Software Orders  | Support  | Contributors  | Advertisers  | 

JCE Print
   Current Issue
 Previous Issues
 Supplements
 Search JCE Index

JCE Digital Library
   ChemInfo
 DigiDemos
 Featured Molecules
 LivTexts
 LrnCom
 QBank
 SymMath
 WebWare

JCE Software
   Latest Releases
 Software & Video
 Downloads
 Support

Only@JCE Online
   JCE Online Store
 JCE HS CLIC
 JCE Discussion Forums
   Biographical Snapshots
 ChemEd Resource Shelf
 Featured Molecules
 Hal's Picks
 Project Chemlab
 Reviewed WWW Sites
 "Web-Ed" Articles

About JCE
   Features
 Publications
 Operations
 Outreach
 Contact Us


  Home > JCE Print > Journal of Chemical Education > Issues > 2006  > February  >
In the Laboratory
Assaying α-Dicarbonyl Compounds in Wine: A Complementary GC–MS, HPLC, and Visible Spectrophotometric Analysis
Tammy J. Dwyer and Jeremiah D. Fillo
Department of Chemistry, University of San Diego, San Diego, CA 92110
Cover
February 2006
Vol. 83 No. 2
p. 273
Abstract
We used a facile, aqueous reaction coupled with gas chromatography–mass spectrometry (GC–MS), visible spectrophotometry, and high performance liquid chromatography (HPLC) to quantify the amounts of α-dicarbonyl compounds in wine samples. The aqueous reaction between an α-dicarbonyl compound and o-phenylenediamine yields a highly stable quinoxaline molecule with an absorbance maximum of 315 nm. GC–MS is used to identify each quinoxaline via mass. Likewise, owing to the similar absorption properties of the quinoxaline products, visible spectrometry is not useful for quantitation although it reveals the absorbance maximum to use for optical detection of the quinoxalines by HPLC. The analytical techniques used in this experiment complement each other since visible spectrophotometry is needed to determine the absorption wavelength, which is used for HPLC analysis; and HPLC is used for quantitation, while GC–MS is used to confirm the identities of the reaction products.
Supplement
Instructions for the students and notes for the instructor are available.
*  Contents JCE2006p0273W.doc (Microsoft Word)
*  Download
JCE2006p0273W.pdf

JCE2006p0273W.zip
More Information
*  Citation
 Dwyer, Tammy J.; Fillo, Jeremiah D. J. Chem. Educ. 2006 83 273.
*  Keywords
 Analytical Chemistry; Aromatic Compounds; Chromatography; Food Science; Hands-On Learning / Manipulatives; HPLC; Laboratory Instruction; Mass Spectrometry; Quantitative Analysis; Second-Year Undergraduate; Synthesis; UV-Vis Spectroscopy
*  History
 Created:
Last Updated:		 1/5/2006
1/9/2006
<< Previous Article Table of Contents Next Article >>
  Home > JCE Print > Journal of Chemical Education > Issues > 2006  > February  > Page 273


Subscriptions
 Subscription Info
 Order Forms

JCE HS CLIC

Our Secondary School editors work hard to distill all the JCE materials to produce a fraction of particular interest to high school teachers. We call it CLIC.
 JCE HS CLIC

Contributions Welcome
JCE welcomes your submission
 Contributors' Corner

Advertisers
In recent years we have worked hard to better match our advertisers with our readers. When shopping for chemistry education materials, visit our advertisers' WWW sites first.
 Recent Advertisers

Be An Ambassador
Take JCE along on your outreach missions. Copies of the Journal, guest access to JCE Online, our publications catalog, and more are available for your participants.
 Outreach with JCE

Comments to
jceonline@chem.wisc.edu		 Copyright © Division of Chemical Education, Inc., American Chemical Society.
All rights reserved.