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  Home > JCE Print > Journal of Chemical Education > Issues > 2006  > February  >
In the Laboratory
The Binding Constant of Estradiol to Bovine Serum Albumin. An Upper-Level Experiment Utilizing Tritium-Labeled Estradiol and Liquid Scintillation Counting
Peihong Liang, Bhavin Adhyaru, Wright L. Pearson, and Kathryn R. Williams
Department of Chemistry, University of Florida, Gainesville, FL 32611-7200
Cover
February 2006
Vol. 83 No. 2
p. 294
Abstract
Students determine the binding constant of estradiol to bovine serum albumin using 3H-labeled estradiol. The estradiol is equilibrated with excess BSA, and subsequently a small fraction (ca. 1%) of the unbound hormone is extracted using solid-phase microextraction (SPME). The fibers are counted using a liquid scintillation counter. The experiment is suitable for laboratories in biophysical chemistry, instrumental analysis, and radiochemistry.
Supplement
Instructor notes and full protocols for the biophysical chemistry and instrumental analysis courses are available.
*  Contents JCE2006p0294W.doc (Microsoft Word)
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*  Citation
 Liang, Peihong; Adhyaru, Bhavin; Pearson, Wright L.; Williams, Kathryn R. J. Chem. Educ. 2006 83 294.
*  Keywords
 Bioanalytical Chemistry; Biophysical Chemistry; Equilibrium; Hands-On Learning / Manipulatives; Hormones; Instrumental Methods; Laboratory Instruction; Nuclear / Radiochemistry; Physical Chemistry; Physical Properties; Proteins / Peptides; Separation Science; Steroids; Upper-Division Undergraduate
*  History
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Last Updated:		 1/5/2006
1/5/2006
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