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  Home > JCE Print > Journal of Chemical Education > Issues > 2006  > May  >
In the Laboratory
DNA Profiling of the D1S80 Locus: A Forensic Analysis for the Undergraduate Biochemistry Laboratory
D. Dewaine Jackson, Chad S. Abbey, and Dylan Nugent
Department of Chemistry, Stetson University, DeLand, FL 32720
Cover
May 2006
Vol. 83 No. 5
p. 774
Abstract
We describe a laboratory exercise in DNA profiling that can be used in an undergraduate biochemistry teaching laboratory to demonstrate four fundamental procedures: isolation of genomic DNA from human (cheek) cells, use of the polymerase chain reaction (PCR) to amplify DNA, separation of amplified DNAs on agarose and polyacrylamide gels, and quantitative analysis of data (while comparing two different gel separation techniques). The target of PCR amplification is the 369–801 base pair D1S80 locus of the human genome, a variable number of tandem repeats (VNTR) locus containing a 16 base pair repeat unit. Students amplify DNA from their entire class, determine the size and genotype of all of the students' D1S80 loci by two gel separation techniques, compare the samples to an "evidence" sample to solve a fictitious crime, and critically evaluate the methodology.
Supplement
Instructions for the students and notes for the instructor are available.
*  Contents JCE2006p0774W.doc (Microsoft Word)
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*  Citation
 Jackson, D. Dewaine; Abbey, Chad S.; Nugent, Dylan. J. Chem. Educ. 2006 83 774.
*  Keywords
 Biochemistry; Biological Cells; Biotechnology; Electrophoresis; First-Year Undergraduate / General; Forensic Chemistry; Hands-On Learning / Manipulatives; Interdisciplinary / Multidisciplinary; Laboratory Instruction; Molecular Biology; Nucleic Acids / DNA / RNA; Quantitative Analysis; Second-Year Undergraduate; Upper-Division Undergraduate
*  History
 Created:
Last Updated:		 3/16/2006
3/31/2006
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