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  Home > JCE Print > Journal of Chemical Education > Issues > 2006  > November  >
In the Laboratory
Cost-Effective Teacher
An Inexpensive Device for Capillary Electrophoresis with Fluorescence Detection
Greg Anderson
Department of Chemistry, Troy State University, Troy, AL 36082

Jonathan E. Thompson and Khriesto Shurrush
Department of Chemistry, University of Nebraska at Kearney, Kearney, NE 68849

Cover
November 2006
Vol. 83 No. 11
p. 1677

Abstract
We describe an inexpensive device for performing capillary electrophoresis (CE) separations with fluorescence detection. As a demonstration of the device's utility we have determined the mass of riboflavin in a commercially available dietary supplement. The device allows for separation of riboflavin in ~100 s with a mass limit of detection of ~10 fmol. The device may be of particular interest to faculty at smaller chemistry departments that do not currently possess a CE instrument. While of limited utility, the device we describe could be used to introduce students to capillary electrophoresis despite the obstacle of limited funding.
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Citation
Anderson, Greg; Thompson, Jonathan E.; Shurrush, Khriesto. J. Chem. Educ. 2006 83 1677.
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Keywords
Analytical Chemistry; Electrophoresis; Fluorescence Spectroscopy; Hands-On Learning / Manipulatives; Instrumental Methods; Laboratory Instruction; Second-Year Undergraduate; Separation Science; Upper-Division Undergraduate
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History
Created:
Last Updated:
9/19/2006
9/20/2006
 Caution! 
Experiments, laboratory exercises, lecture demonstrations, and other descriptions of the use of chemicals, apparatus, instruments, computers, and computer interfaces are presented in the Journal of Chemical Education as illustrative of new or improved ideas or concepts in chemistry instruction and are directed at qualified teachers. Although every effort is made to assure and encourage safe practices and safe use of chemicals, the Journal of Chemical Education cannot assume responsibility for uses made of its published materials. Many chemicals are hazardous. Precautions for the safe use of hazardous chemicals and directions for their proper disposal are described in the Material Safety Data Sheets and on the labels. We strongly urge all those planning to use materials from our pages to make choices and to develop procedures for laboratory and classroom safety in accordance with local needs and situations.
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