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  Home > JCE Print > Journal of Chemical Education > Issues > 2006  > September  >
In the Laboratory
Spectroscopic Measurement of the Redox Potential of Cytochrome c for the Undergraduate Biochemistry Laboratory
Douglas B. Craig
Department of Chemistry, University of Winnipeg, Winnipeg, Manitoba, Canada R3B 2E9

Ellert R. Nichols
Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada R3E 0W3

Cover
September 2006
Vol. 83 No. 9
p. 1325

Abstract
Teaching redox chemistry is an important facet of undergraduate biochemistry courses, particularly with respect to developing the students' understanding of the central metabolic processes such as the mitochondrial respiratory chain and photosynthesis. We have introduced an experiment for the undergraduate metabolism course involving the spectroscopic measurement of the redox potential of cytochrome c. The experiment permits a quantitative result within a single three-hour laboratory period and complements the basic redox concepts and calculations shown in class.
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Instructions for the students and notes for the instructor are available.
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Citation
Craig, Douglas B.; Nichols, Ellert R. J. Chem. Educ. 2006 83 1325.
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Keywords
Bioanalytical Chemistry; Biochemistry; Electrochemistry; Hands-On Learning / Manipulatives; Laboratory Instruction; Upper-Division Undergraduate; UV-Vis Spectroscopy
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History
Created:
Last Updated:
8/7/2006
8/18/2006
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Experiments, laboratory exercises, lecture demonstrations, and other descriptions of the use of chemicals, apparatus, instruments, computers, and computer interfaces are presented in the Journal of Chemical Education as illustrative of new or improved ideas or concepts in chemistry instruction and are directed at qualified teachers. Although every effort is made to assure and encourage safe practices and safe use of chemicals, the Journal of Chemical Education cannot assume responsibility for uses made of its published materials. Many chemicals are hazardous. Precautions for the safe use of hazardous chemicals and directions for their proper disposal are described in the Material Safety Data Sheets and on the labels. We strongly urge all those planning to use materials from our pages to make choices and to develop procedures for laboratory and classroom safety in accordance with local needs and situations.
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