JCE Online Journal of Chemical EducationDivision of Chemical Education, American Chemical SocietyAmerican Chemical Society
 | Subscriptions  | Software Orders  | Support  | Contributors  | Advertisers  | 

JCE Print

JCE Digital Library

JCE Software

Only@JCE Online

About JCE


  Home > JCE Print > Journal of Chemical Education > Issues > 2007  > April  >
In the Laboratory
Homemade Bienzymatic-Amperometric Biosensor for Beverages Analysis
M. C. Blanco-López, M. J. Lobo-Castañón, and A. J. Miranda-Ordieres
Departamento de Química Física y Analítica, Facultad de Química, Universidad de Oviedo, 33006 Oviedo, Spain
Cover
April 2007
Vol. 84 No. 4
p. 677

Abstract
In this experiment students construct an amperometric glucose biosensor and use it to determine the glucose concentration in soft drinks. This experiment is appropriate for an advanced instrumental course; however, it could be adapted to elementary levels. The design is based on the immobilization of the glucose oxidase and horseradish peroxidase enzymes with a redox mediator (ferrocene) in a carbon paste electrode. The glucose concentration is directly related to the cathodic current yielded by the oxidized form of the mediator obtained in the enzymatic reactions. The students use cyclic voltammetry to optimize sensor design and performance, and analytical characterization and glucose determination is carried out amperometrically. The preparation of the sensor is simple, quick, and can be carried out with inexpensive materials. The challenge of constructing biosensing devices and the good results typically obtained are very stimulating for the undergraduate students.
Supplement
Instructions for the students and notes for the instructor are available.
*
Download
Contents
More Information
*
Citation
Blanco-López, M. C.; Lobo-Castañón, M. J.; Miranda-Ordieres, A. J. J. Chem. Educ. 2007, 84, 677.
*
Keywords
Analytical Chemistry; Bioanalytical Chemistry; Electrochemistry; Enzymes; Food Science; Hands-On Learning / Manipulatives; Laboratory Instruction; Oxidation / Reduction; Upper-Division Undergraduate
*
History
Created:
Last Updated:
3/6/2007
3/8/2007
 Caution! 
Experiments, laboratory exercises, lecture demonstrations, and other descriptions of the use of chemicals, apparatus, instruments, computers, and computer interfaces are presented in the Journal of Chemical Education as illustrative of new or improved ideas or concepts in chemistry instruction and are directed at qualified teachers. Although every effort is made to assure and encourage safe practices and safe use of chemicals, the Journal of Chemical Education cannot assume responsibility for uses made of its published materials. Many chemicals are hazardous. Precautions for the safe use of hazardous chemicals and directions for their proper disposal are described in the Material Safety Data Sheets and on the labels. We strongly urge all those planning to use materials from our pages to make choices and to develop procedures for laboratory and classroom safety in accordance with local needs and situations.
  Home > JCE Print > Journal of Chemical Education > Issues > 2007  > April  > Page 677


Subscriptions

JCE HS CLIC

Our Secondary School editors work hard to distill all the JCE materials to produce a fraction of particular interest to high school teachers. We call it CLIC.


Contributions Welcome
JCE welcomes your submission

Advertisers
In recent years we have worked hard to better match our advertisers with our readers. When shopping for chemistry education materials, visit our advertisers' WWW sites first.

Be An Ambassador
Take JCE along on your outreach missions. Copies of the Journal, guest access to JCE Online, our publications catalog, and more are available for your participants.