JCE Online Journal of Chemical EducationDivision of Chemical Education, American Chemical SocietyAmerican Chemical Society
 | Subscriptions  | Software Orders  | Support  | Contributors  | Advertisers  | 

JCE Print

JCE Digital Library

JCE Software

Only@JCE Online

About JCE



  Home > JCE Print > Journal of Chemical Education > Issues > 2007  > December  >
In the Laboratory
High-Pressure Liquid Chromatography: Quantitative Analysis of Chinese Herbal Medicine
W. F. Chan and C. W. Lin
Department of Chemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong
Cover
December 2007
Vol. 84 No. 12
p. 1982

Abstract
An HPLC undergraduate experiment on the analysis of traditional Chinese medicine (TCM) has been developed. Two commonly used herbs (glycyrrhizae radix and cinnamomi ramulus) are studied. Glycyrrhizin, cinnamic acid, and cinnamaldehyde are chosen as markers for the herbs. The dried herbs in their natural state and a TCM preparation in powder form are analyzed. Gradient elution with diode-array detection is used for the separation and identification of the markers. Solid-phase extraction is employed for cleaning the sample solutions.
Supplement
Handouts for the students, notes for the instructors, and spectra are available.
*
Download
Contents
More Information
*
Citation
Chan, W. F.; Lin, C. W. J. Chem. Educ. 2007, 84, 1982.
*
Keywords
Analytical Chemistry; Drugs / Pharmaceuticals; Hands-On Learning / Manipulatives; HPLC; Laboratory Instruction; Medicinal Chemistry; Natural Products; Quantitative Analysis; Separation Science; Upper-Division Undergraduate
*
History
Created:
Last Updated:
10/25/2007
10/31/2007
 Caution! 
Experiments, laboratory exercises, lecture demonstrations, and other descriptions of the use of chemicals, apparatus, instruments, computers, and computer interfaces are presented in the Journal of Chemical Education as illustrative of new or improved ideas or concepts in chemistry instruction and are directed at qualified teachers. Although every effort is made to assure and encourage safe practices and safe use of chemicals, the Journal of Chemical Education cannot assume responsibility for uses made of its published materials. Many chemicals are hazardous. Precautions for the safe use of hazardous chemicals and directions for their proper disposal are described in the Material Safety Data Sheets and on the labels. We strongly urge all those planning to use materials from our pages to make choices and to develop procedures for laboratory and classroom safety in accordance with local needs and situations.
  Home > JCE Print > Journal of Chemical Education > Issues > 2007  > December  > Page 1982


Subscriptions

JCE HS CLIC

Our Secondary School editors work hard to distill all the JCE materials to produce a fraction of particular interest to high school teachers. We call it CLIC.


Contributions Welcome
JCE welcomes your submission

Advertisers
In recent years we have worked hard to better match our advertisers with our readers. When shopping for chemistry education materials, visit our advertisers' WWW sites first.

Be An Ambassador
Take JCE along on your outreach missions. Copies of the Journal, guest access to JCE Online, our publications catalog, and more are available for your participants.