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| Home > JCE Print > Journal of Chemical Education > Issues >
2007
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January
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In the Laboratory
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Manual Solid–Phase Peptide Synthesis of Metallocene–Peptide Bioconjugates
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Srecko I. Kirin, Fozia Noor, and Nils Metzler–Nolte
Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, D-69120 Heidelberg, Germany
Walter Mier
Department of Nuclear Medicine, University Hospital Heidelberg, D-69120 Heidelberg, Germany
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January 2007
Vol. 84 No. 1
p. 108
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| Abstract |
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We describe a simple procedure for the manual solid-phase synthesis of peptides. No expensive or specialized equipment is needed. Moreover, a variety of markers can be attached to the N-terminus. We describe two organometallic markers and a fluroescent dye, in addition to an unlabeled derivative that serves as a comparison. The experiment described herein was successfully carried out by fourth-year undergraduate students of molecular biotechnology. By carrying out this experiment, the students gained first-hand experience for one of the most important modern synthesis techniques.
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| Supplement |
Student spreadsheets, instructor notes, a list of chemicals and equipment, hazards and safety instructions, reactions schemes and mechanisms, and ESI–MS of the products are available.
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| More Information |
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Citation |
Kirin, Srecko I.; Noor, Fozia; Metzler-Nolte, Nils; Mier, Water. J. Chem. Educ. 2007, 84, 108.
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Keywords |
bioorganometallic chemistry; metallocenes; peptide synthesis; peptides; solid phase synthesis
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History |
Created:
Last Updated: |
12/5/2006
3/20/2007
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 Caution! | | Experiments, laboratory exercises, lecture demonstrations, and other descriptions of the use of chemicals, apparatus, instruments, computers, and computer interfaces are presented in the Journal of Chemical Education as illustrative of new or improved ideas or concepts in chemistry instruction and are directed at qualified teachers. Although every effort is made to assure and encourage safe practices and safe use of chemicals, the Journal of Chemical Education cannot assume responsibility for uses made of its published materials. Many chemicals are hazardous. Precautions for the safe use of hazardous chemicals and directions for their proper disposal are described in the Material Safety Data Sheets and on the labels. We strongly urge all those planning to use materials from our pages to make choices and to develop procedures for laboratory and classroom safety in accordance with local needs and situations. |
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2007
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January
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108
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