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  Home > JCE Print > Journal of Chemical Education > Issues > 2007  > January  >
In the Laboratory
Survey of Biochemical Separation Techniques
Melanie R. Nilsson
Department of Chemistry, McDaniel College, Westminster, MD 21157-4390
Cover
January 2007
Vol. 84 No. 1
p. 112

Abstract
The separation of a mixture of myoglobin and blue dextran is explored using five different biochemical separation techniques (syringe filtration, Centricon, dialysis, gel filtration, and solid-phase extraction). The mixture is green and separation is detected by the visual observation of the colored components (orange–red myoglobin and blue dextran). Students gain exposure and hands-on practice with a broad range of modern biochemical separation techniques and the entire exercise can easily be completed in one laboratory period.
Supplement
Instructions for the students and notes for the instructor are available.
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Citation
Nilsson, Melanie R. J. Chem. Educ. 2007, 84, 112.
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Keywords
Biochemistry; Chromatography; Laboratory Instruction; Proteins / Peptides; Separation Science; Upper-Division Undergraduate
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History
Created:
Last Updated:
12/5/2006
3/20/2007
 Caution! 
Experiments, laboratory exercises, lecture demonstrations, and other descriptions of the use of chemicals, apparatus, instruments, computers, and computer interfaces are presented in the Journal of Chemical Education as illustrative of new or improved ideas or concepts in chemistry instruction and are directed at qualified teachers. Although every effort is made to assure and encourage safe practices and safe use of chemicals, the Journal of Chemical Education cannot assume responsibility for uses made of its published materials. Many chemicals are hazardous. Precautions for the safe use of hazardous chemicals and directions for their proper disposal are described in the Material Safety Data Sheets and on the labels. We strongly urge all those planning to use materials from our pages to make choices and to develop procedures for laboratory and classroom safety in accordance with local needs and situations.
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