JCE Online Journal of Chemical EducationDivision of Chemical Education, American Chemical SocietyAmerican Chemical Society
 | Subscriptions  | Software Orders  | Support  | Contributors  | Advertisers  | 

JCE Print
   Current Issue
 Previous Issues
 Supplements
 Search JCE Index

JCE Digital Library
   ChemInfo
 DigiDemos
 Featured Molecules
 LivTexts
 LrnCom
 QBank
 SymMath
 WebWare

JCE Software
   Latest Releases
 Software & Video
 Downloads
 Support

Only@JCE Online
   JCE Online Store
 JCE HS CLIC
 JCE Discussion Forums
   Biographical Snapshots
 ChemEd Resource Shelf
 Featured Molecules
 Hal's Picks
 Project Chemlab
 Reviewed WWW Sites
 "Web-Ed" Articles

About JCE
   Features
 Publications
 Operations
 Outreach
 Contact Us


  Home > JCE Print > Journal of Chemical Education > Issues > 2007  > July  >
In the Laboratory
Absorption and Scattering Coefficients: A Biophysical-Chemistry Experiment Using Reflectance Spectroscopy
Gabriela B. Cordon and M. Gabriela Lagorio
INQUIMAE / Departamento de Química Inorgánica, Analítica y Química Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
Cover
July 2007
Vol. 84 No. 7
p. 1167
Abstract
The scattering, S, and the absorption, K, coefficients of leaves from different plant species were determined separately as a function of wavelength from reflectance and transmittance data measured for the individual leaves. The equations that link the measurable data with the optical parameters K and S, were deduced using both the Kubelka–Munk approach and the model of the "pile of plates". In parallel, the reflectance of an optically thick sample of stacked leaves were measured and the remission function was calculated. The values of the remission function obtained at different wavelengths from stacked leaves were compared with the quotients of the optical parameters, K/S, obtained from individual leaves. Both values are predicted to be equal according the Kubelka–Munk theory. The experimental agreement shows that the pile of plates model is a good description for the optical properties of leaves.
Supplement
Instructions for the students and notes for the instructor are available.
*
 Download
Contents
  JCE2007p1167W.pdf
 
JCE2007p1167W.zip
JCE2007p1167W.doc (Microsoft Word)
More Information
*
Citation
Cordon, Gabriela B.; Lagorio, M. Gabriela. J. Chem. Educ. 2007, 84, 1167.
*
Keywords
Agricultural Chemistry; Biophysical Chemistry; Graduate Education / Research; Hands-On Learning / Manipulatives; Instrumental Methods; Laboratory Instruction; Physical Chemistry; Physical Properties; Plant Chemistry; Spectroscopy; Upper-Division Undergraduate; UV-Vis Spectroscopy
*
History
Created:
Last Updated:
5/29/2007
6/7/2007
<< Previous Article Table of Contents Next Article >>
  Home > JCE Print > Journal of Chemical Education > Issues > 2007  > July  > Page 1167


Subscriptions
 Subscription Info
 Order Forms

JCE HS CLIC

Our Secondary School editors work hard to distill all the JCE materials to produce a fraction of particular interest to high school teachers. We call it CLIC.
 JCE HS CLIC

Contributions Welcome
JCE welcomes your submission
 Contributors' Corner

Advertisers
In recent years we have worked hard to better match our advertisers with our readers. When shopping for chemistry education materials, visit our advertisers' WWW sites first.
 Recent Advertisers

Be An Ambassador
Take JCE along on your outreach missions. Copies of the Journal, guest access to JCE Online, our publications catalog, and more are available for your participants.
 Outreach with JCE

Comments to
jceonline@chem.wisc.edu		 Copyright © Division of Chemical Education, Inc., American Chemical Society.
All rights reserved.