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2007
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In the Laboratory
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Synthesis of Plant Auxin Derivatives and Their Effects on Ceratopteris richardii
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A Collaborative Experiment between Undergraduate Organic and Biochemistry Laboratories
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Corey E. Stilts and Roxanne Fisher
Department of Chemistry, Chatham College, Pittsburgh, PA 15232
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June 2007
Vol. 84 No. 6
p. 999
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| Abstract |
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In this experiment, organic chemistry laboratory students synthesized derivatives of their own design of the plant auxins indole-3-acetic acid (IAA) and 1-naphthalene acetic acid (NAA). Their syntheses varied from making ester to amide derivatives. After optimizing their synthesis, the organic chemistry student groups shared their products with a student group from the biochemistry–cell biology lab. The biochemistry–cell biology students compared the development of Ceratopteris richardii gametophytes on three types of sterile medium: medium without hormones, medium with 10-5 M NAA, and medium with 10-4 M of an auxin derivative synthesized in the organic chemistry lab. The organic syntheses were performed over a four-week period, and observations of C. richardii gametophytes were done for a two week period, after which the gametophyte measurements where analyzed quantitatively using Microsoft Excel. The project terminated in a poster session presented by both the organic chemistry and biochemistry–cell biology classes during which the students discussed their results and compared any structure activity relationships that were evident. Results from this bioassay of auxin derivatives typically fall into four groups: (i) there is an effect on gametophyte growth similar to that of known auxins, (ii) there is no effect on the growth of the gametophyte, (iii) there is a novel effect on gametophyte growth, or (iv) the auxin derivative is lethal to the gametophyte.
Featured on the Cover.
See JCE Featured Molecules.
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A detailed handout to students, background material, preparation details, grading sheets as well as further information for instructors are available.
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| More Information |
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Citation |
Stilts, Corey E.; Fisher, Roxanne. J. Chem. Educ. 2007, 84, 999.
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Keywords |
Biochemistry; Biological Cells; Bioorganic Chemistry; Collaborative / Cooperative Learning; Communication / Writing; Hands-On Learning / Manipulatives; Hormones; Inquiry-Based / Discovery Learning; Interdisciplinary / Multidisciplinary; Laboratory Instruction; Organic Chemistry; Problem Solving / Decision Making; Second-Year Undergraduate; Synthesis; Upper-Division Undergraduate
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History |
Created:
Last Updated: |
4/24/2007
5/3/2007
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 Caution! | | Experiments, laboratory exercises, lecture demonstrations, and other descriptions of the use of chemicals, apparatus, instruments, computers, and computer interfaces are presented in the Journal of Chemical Education as illustrative of new or improved ideas or concepts in chemistry instruction and are directed at qualified teachers. Although every effort is made to assure and encourage safe practices and safe use of chemicals, the Journal of Chemical Education cannot assume responsibility for uses made of its published materials. Many chemicals are hazardous. Precautions for the safe use of hazardous chemicals and directions for their proper disposal are described in the Material Safety Data Sheets and on the labels. We strongly urge all those planning to use materials from our pages to make choices and to develop procedures for laboratory and classroom safety in accordance with local needs and situations. |
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