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2008
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In the Laboratory
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HPLC Analysis of α- and β-Acids in Hops
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Travis M. Danenhower, Leyna J. Force, Kenneth J. Petersen, Thomas A. Betts
Department of Physical Sciences, Kutztown University of PA, Kutztown, PA 19530-0730
Gary A. Baker
Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6110
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July 2008
Vol. 85 No. 7
p. 954
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| Abstract |
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Hops have been used for centuries to impart aroma and bitterness to beer. The cones of the female hop plant contain both essential oils, which include many of the fragrant components of hops, and a collection of compounds known as α- and β-acids that are the precursors to bittering agents. In order for brewers to predict the ultimate bitterness of a brewed product, it is important to have an accurate assessment of the α- and β-acid content of the hops. A high-performance liquid chromatography method is described here for the quantitation of the predominant α-acids (humulone, cohumulone, and adhumulone) and β-acids (lupulone, colupulone, and adlupulone) present in hops. Additional laboratory projects are suggested that allow students to explore various aspects of method assessment and validation.
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| Supplement |
Student handouts; Instructor notes including sample chromatograms of α- and β-acid separations on various HPLC columns along with details and results of the quality assurance and method validation extensions
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| More Information |
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Citation |
Danenhower, Travis M.; Force, Leyna A.; Petersen, Kenneth J.; Betts, Thomas A.; Baker, Gary A. J. Chem. Educ. 2008, 85, 954.
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Keywords |
Analytical Chemistry; Chromatography; Food Science; Hands-On Learning / Manipulatives; HPLC; Instrumental Methods; Laboratory Instruction; Plant Chemistry; Quantitative Analysis; Second-Year Undergraduate; Separation Science; Upper-Division Undergraduate
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History |
Created:
Last Updated: |
5/27/2008
6/4/2008
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 Caution! | | Experiments, laboratory exercises, lecture demonstrations, and other descriptions of the use of chemicals, apparatus, instruments, computers, and computer interfaces are presented in the Journal of Chemical Education as illustrative of new or improved ideas or concepts in chemistry instruction and are directed at qualified teachers. Although every effort is made to assure and encourage safe practices and safe use of chemicals, the Journal of Chemical Education cannot assume responsibility for uses made of its published materials. Many chemicals are hazardous. Precautions for the safe use of hazardous chemicals and directions for their proper disposal are described in the Material Safety Data Sheets and on the labels. We strongly urge all those planning to use materials from our pages to make choices and to develop procedures for laboratory and classroom safety in accordance with local needs and situations. |
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