JCE Online Journal of Chemical EducationDivision of Chemical Education, American Chemical SocietyAmerican Chemical Society
 | Subscriptions  | Software Orders  | Support  | Contributors  | Advertisers  | 

JCE Print

JCE Digital Library

JCE Software

Only@JCE Online

About JCE



  Home > JCE Print > Journal of Chemical Education > Issues > 2008  > March  >
In the Laboratory
Measurement of Iron in Egg Yolk: An Instrumental Analysis Experiment Using Biochemical Principles
Kevin M. Maloney, Emmanuel M. Quiazon, and Ramee Indralingam
Department of Chemistry, Stetson University, DeLand, FL 32723
Cover
March 2008
Vol. 85 No. 3
p. 399

Abstract
The generally accepted method to determine iron content in food is by acid digestion or dry ashing and subsequent flame atomic absorption spectrometry or inductively coupled plasma atomic emission spectrometry. We have developed an experiment that chemically extracts the iron from an egg yolk and quantifies it using UV–vis absorption spectrophotometry. Only the yolk of an egg is used because it contains almost all of the iron in an egg and the extraction is more efficient than with the whole egg. The experiment is suitable for the instrumental analysis portion of the analytical chemistry course and serves to demonstrate to the students the chemical and biochemical principles that can be used for the extraction of a mineral from a complex organic matrix.
Supplement
Student handouts; Instructor notes
*
Download
Contents
More Information
*
Citation
Maloney, Kevin M.; Quiazon, Emmanuel M.; Indralingam, Ramee. J. Chem. Educ. 2008, 85, 399.
*
Keywords
Analytical Chemistry; Bioanalytical Chemistry; Food Science; Hands-On Learning / Manipulatives; Instrumental Methods; Laboratory Instruction; Upper-Division Undergraduate; UV-Vis Spectroscopy
*
History
Created:
Last Updated:
2/4/2008
2/8/2008
 Caution! 
Experiments, laboratory exercises, lecture demonstrations, and other descriptions of the use of chemicals, apparatus, instruments, computers, and computer interfaces are presented in the Journal of Chemical Education as illustrative of new or improved ideas or concepts in chemistry instruction and are directed at qualified teachers. Although every effort is made to assure and encourage safe practices and safe use of chemicals, the Journal of Chemical Education cannot assume responsibility for uses made of its published materials. Many chemicals are hazardous. Precautions for the safe use of hazardous chemicals and directions for their proper disposal are described in the Material Safety Data Sheets and on the labels. We strongly urge all those planning to use materials from our pages to make choices and to develop procedures for laboratory and classroom safety in accordance with local needs and situations.
  Home > JCE Print > Journal of Chemical Education > Issues > 2008  > March  > Page 399


Subscriptions

JCE HS CLIC

Our Secondary School editors work hard to distill all the JCE materials to produce a fraction of particular interest to high school teachers. We call it CLIC.


Contributions Welcome
JCE welcomes your submission

Advertisers
In recent years we have worked hard to better match our advertisers with our readers. When shopping for chemistry education materials, visit our advertisers' WWW sites first.

Be An Ambassador
Take JCE along on your outreach missions. Copies of the Journal, guest access to JCE Online, our publications catalog, and more are available for your participants.