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  Home > JCE Print > Journal of Chemical Education > Issues > 2008  > May  >
In the Laboratory
A Simple Laboratory Experiment To Determine the Kinetics of Mutarotation of D-Glucose Using a Blood Glucose Meter
Carlos E. Perles and Pedro L. O. Volpe
Institute of Chemistry, State University of Campinas, CEP 13084-971 Campinas, São Paulo, Brazil
Cover
May 2008
Vol. 85 No. 5
p. 686

Abstract
A simple commercial blood glucose meter is used to follow the kinetics of mutarotation of D-glucose in aqueous solution. The results may be compared with those obtained using an automatic polarimeter, if this is available This experiment is proposed for use by students in a general chemistry, biology, organic chemistry, and physical chemistry laboratory at the college level. It can also be used as an alternative technique to the classical methods that use relatively expensive equipment, which are not always accessible to students. The technique of indirect measurement of the kinetics of mutarotation of D-glucose through an enzymatic redox reaction also provides an example of the chemical and kinetic approaches to the mutarotation phenomenon and the principle of enzymatic stereospecificity. The test strips of the blood glucose meter contain glucose dehydrogenase, an enzyme specific for the anomer of D-glucose in solution.
Supplement
Experimetal procedure and post-laboratory questions; Instructor notes
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Citation
Perles, Carlos E.; Volpe, Pedro L. O. J. Chem. Educ. 2008, 85, 686.
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Keywords
Analytical Chemistry; Aqueous Solution Chemistry; Bioanalytical Chemistry; Biochemistry; Carbohydrates; Chirality / Optical Activity; Enzymes; First-Year Undergraduate / General; Hands-On Learning / Manipulatives; Kinetics; Laboratory Instruction; Physical Chemistry; Solutions / Solvents; Stereochemistry
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History
Created:
Last Updated:
3/25/2008
3/28/2008
 Caution! 
Experiments, laboratory exercises, lecture demonstrations, and other descriptions of the use of chemicals, apparatus, instruments, computers, and computer interfaces are presented in the Journal of Chemical Education as illustrative of new or improved ideas or concepts in chemistry instruction and are directed at qualified teachers. Although every effort is made to assure and encourage safe practices and safe use of chemicals, the Journal of Chemical Education cannot assume responsibility for uses made of its published materials. Many chemicals are hazardous. Precautions for the safe use of hazardous chemicals and directions for their proper disposal are described in the Material Safety Data Sheets and on the labels. We strongly urge all those planning to use materials from our pages to make choices and to develop procedures for laboratory and classroom safety in accordance with local needs and situations.
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