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  Home > JCE Print > Journal of Chemical Education > Issues > 2008  > September  >
In the Laboratory
Cost-Effective Teacher
A Computer-Interfaced Drop Counter as an Inexpensive Fraction Collector for Column Chromatography
Barbara T. Nash
Division of Natural Sciences and Veterinary Technology, Mercy College, Dobbs Ferry, NY
Cover
September 2008
Vol. 85 No. 9
p. 1260

Abstract
A computer-interfaced drop counter is described that serves as an inexpensive alternative to a fraction collector for column chromatography experiments. Undergraduate biochemistry laboratories frequently do not have the budget to purchase fraction collectors. Protocols that call for the manual measurement of fraction volumes as well as the manual application of buffer to the column do not provide students with the skills required to function in a professional setting. In this experiment, students use a column outfitted with Luer fittings to set up an automatic buffer-feeding system and also monitor fraction collection using a drop counter. Students have the opportunity to learn troubleshooting techniques for both methods. To illustrate this system, a sample of typical student data from an experiment in which the molecular weight of myoglobin is determined by gel filtration chromatography is shown. The system described can be used for any column chromatography experiment, including preparative gel filtration chromatography, ion exchange chromatography, and affinity chromatography.
Supplement
Protocol for the experiment used to generate the data; Student handout; Instructor notes including answers to a post-lab problem set; Results of a student survey
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Citation
Nash, Barbara T. J. Chem. Educ. 2008, 85, 1260.
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Keywords
Analytical Chemistry; Bioanalytical Chemistry; Biochemistry; Chromatography; Computer-Based Learning; Hands-On Learning / Manipulatives; Laboratory Computing / Interfacing; Laboratory Equipment / Apparatus; Laboratory Instruction; Physical Properties; Proteins / Peptides; Upper-Division Undergraduate
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History
Created:
Last Updated:
8/4/2008
8/12/2008
 Caution! 
Experiments, laboratory exercises, lecture demonstrations, and other descriptions of the use of chemicals, apparatus, instruments, computers, and computer interfaces are presented in the Journal of Chemical Education as illustrative of new or improved ideas or concepts in chemistry instruction and are directed at qualified teachers. Although every effort is made to assure and encourage safe practices and safe use of chemicals, the Journal of Chemical Education cannot assume responsibility for uses made of its published materials. Many chemicals are hazardous. Precautions for the safe use of hazardous chemicals and directions for their proper disposal are described in the Material Safety Data Sheets and on the labels. We strongly urge all those planning to use materials from our pages to make choices and to develop procedures for laboratory and classroom safety in accordance with local needs and situations.
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