JCE Online Journal of Chemical EducationDivision of Chemical Education, American Chemical SocietyAmerican Chemical Society
 | Subscriptions  | Software Orders  | Support  | Contributors  | Advertisers  | 

JCE Print

JCE Digital Library

JCE Software

Only@JCE Online

About JCE


  Home > JCE Print > Journal of Chemical Education > Issues > 2008  > September  >
In the Laboratory
Optimizing Chromatographic Separation: An Experiment Using an HPLC Simulator
R. A. Shalliker, S. Kayillo, and G. R. Dennis
Nanoscale Organization and Dynamics Group, School of Natural Sciences, University of Western Sydney, NSW, Australia
Cover
September 2008
Vol. 85 No. 9
p. 1265

Abstract
Optimization of a chromatographic separation within the time constraints of a laboratory session is practically impossible. However, by employing a HPLC simulator, experiments can be designed that allow students to develop an appreciation of the complexities involved in optimization procedures. In the present exercise, a HPLC simulator from JCE Software is used to optimize the separation of a nine-component mixture. The relationship between solvent composition and retention factor is measured to determine the solvent composition for maximized separation. Use of Excel spreadsheets assists in the analysis of the data required for the optimization process. As a result, this exercise demonstrates the basics of the iterative process for optimization of separations and application of spreadsheets to solve relatively complex and tedious repetitive problems.
Supplement
Student handouts and instructor notes; A typical spreadsheet for Excel 2003
*
Download
Contents
More Information
*
Citation
Shalliker, R. A.; Kayillo, S.; Dennis, G. R. J. Chem. Educ. 2008, 85, 1265.
*
Keywords
Analytical Chemistry; Aromatic Compounds; Chromatography; Computer-Based Learning; Hands-On Learning / Manipulatives; Laboratory Instruction; Separation Science; Upper-Division Undergraduate
*
History
Created:
Last Updated:
8/4/2008
8/12/2008
 Caution! 
Experiments, laboratory exercises, lecture demonstrations, and other descriptions of the use of chemicals, apparatus, instruments, computers, and computer interfaces are presented in the Journal of Chemical Education as illustrative of new or improved ideas or concepts in chemistry instruction and are directed at qualified teachers. Although every effort is made to assure and encourage safe practices and safe use of chemicals, the Journal of Chemical Education cannot assume responsibility for uses made of its published materials. Many chemicals are hazardous. Precautions for the safe use of hazardous chemicals and directions for their proper disposal are described in the Material Safety Data Sheets and on the labels. We strongly urge all those planning to use materials from our pages to make choices and to develop procedures for laboratory and classroom safety in accordance with local needs and situations.
  Home > JCE Print > Journal of Chemical Education > Issues > 2008  > September  > Page 1265


Subscriptions

JCE HS CLIC

Our Secondary School editors work hard to distill all the JCE materials to produce a fraction of particular interest to high school teachers. We call it CLIC.


Contributions Welcome
JCE welcomes your submission

Advertisers
In recent years we have worked hard to better match our advertisers with our readers. When shopping for chemistry education materials, visit our advertisers' WWW sites first.

Be An Ambassador
Take JCE along on your outreach missions. Copies of the Journal, guest access to JCE Online, our publications catalog, and more are available for your participants.