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  Home > JCE Print > Journal of Chemical Education > Issues > 2005  > April  >
In the Laboratory
Testing for Genetically Modified Foods Using PCR
Ann Taylor
Department of Chemistry, Wabash College, Crawfordsville, IN 47933

Samin Sajan
Department of Biology and Biomedical Sciences, Washington University in St. Louis, St. Louis, MO 63130


Cover
April 2005
Vol. 82 No. 4
p. 597

Abstract
The polymerase chain reaction (PCR) is a powerful technique used to detect and amplify a specific DNA sequence. In this experiment, DNA is isolated from commercially available corn meal and corn-muffin mixes, then a sequence unique to transgenic plants is amplified by PCR. The amplified sequence is identified by its size (192 base pairs) by agarose gel electrophoresis. This experiment could be used in either a biochemistry course or in an analytical chemistry course in a curriculum that integrates biochemistry throughout the course work.
Supplement
A student handout including background information as well as detailed instructions for the instructor are available. Also included is another experiment for genetically modified food products, and information for a general and an advanced biochemistry version with teaching notes and answer keys .
*  Contents Folder JCE2005p0597W containing JCE2005p0579W_1.doc, JCE2005p0597W_2.doc, JCE2005p0597W_3.doc, JCE2005p0597W_4.doc, JCE2005p0597W_5.doc, and JCE2005p0597W_6.doc (Microsoft Word).
*  Download
JCE2005p0597W.pdf

JCE2005p0597W.zip

More Information
*  Citation
Taylor, Ann; Sajan, Samin. J. Chem. Educ. 2005 82 597.
*  Keywords
Agricultural Chemistry; Biochemistry; Consumer Chemistry; Genetics / Genetic Engineering; Nucleic Acids / DNA / RNA; Plant Chemistry
*  History
Created:
Last Updated:
March 4, 2005
March 14, 2005
  Home > JCE Print > Journal of Chemical Education > Issues > 2005  > April  > Page 597


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