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  Home > JCE Print > Journal of Chemical Education > Issues > 2005  > January  >
In the Laboratory
Equilibrium Gel Filtration Chromatography for the Measurement of Protein–Ligand Binding in the Undergraduate Biochemistry Laboratory
Douglas B. Craig
Department of Chemistry, University of Winnipeg, Winnipeg, MB R3B 2E9, Canada
Cover
January 2005
Vol. 82 No. 1
p. 96
Abstract
Equilibrium gel filtration liquid chromatography is a technique used for the quantitation of ligand binding. We have designed an experiment for the senior undergraduate laboratory using this technique for the measurement of the binding of ATP to cytochrome c. This experiment introduces the student to the use of liquid chromatography and absorbance spectrometry and permits a quantitative result within a three-hour period. The experiment complements classroom discussions regarding liquid chromatography, spectroscopy, dynamic equilibrium, and kinetic parameters.
Supplement
Instructions for the students and notes for the instructor are available.
*  Contents JCE2005p0096W.doc (Microsoft Word)
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More Information
*  Citation
 Craig, Douglas B. J. Chem. Educ. 2005 82 96.
*  Keywords
 Biochemistry; Chromatography; Laboratory Instruction; Proteins / Peptides; Quantitative Analysis; UV-Vis Spectroscopy
*  History
 Created:
Last Updated:		 November 29, 2004
December 8, 2004
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