JCE Online Journal of Chemical Education
 | Subscriptions  | Software Orders  | Support  | Contributors  | Advertisers  | 

JCE Print
   Current Issue
 Previous Issues
 Supplements
 Search JCE Index

JCE Digital Library
   DigiDemos
 QBank
 SymMath
 WebWare

JCE Software
   Latest Releases
 Software & Video
 Downloads
 Support

Only@JCE Online
   JCE Online Store
 JCE HS CLIC
 JCE Discussion Forums
   Biographical Snapshots
 ChemEd Resource Shelf
 Featured Molecules
 Hal's Picks
 Project Chemlab
 Reviewed WWW Sites
 "Web-Ed" Articles

About JCE
   Features
 Publications
 Operations
 Outreach
 Contact Us


  Home > JCE Print > Journal of Chemical Education > Issues > 2005  > January  >
In the Laboratory
Fundamentals of Biomolecule Analysis by Electrospray Ionization Mass Spectrometry. An Instrumental Analysis Laboratory Experiment
Andrea Weinecke and Victor Ryzhov
Department of Chemistry and Biochemistry, Northern Illinois University, DeKalb, IL 60115
Cover
January 2005
Vol. 82 No. 1
p. 99
Abstract
This article describes an undergraduate instrumental analysis laboratory experiment dealing with fundamentals of electrospray ionization mass spectrometry (ESI–MS). Several important aspects of applications of ESI–MS for analysis of biomolecules are addressed. First, the formation of multiply charged ions and deconvolution of such ESI spectra to yield the molecular mass of the analyte is discussed. Second, the effects of isotopic contribution (mostly of 13C) are observed experimentally and verified by calculations. Last, the resolution of a mass spectrometer is probed as the ability to resolve individual peaks in isotopic clusters. Although the experiment is described for quadrupole ion trap mass spectrometers (two major manufacturers’ machines are tested here), it can be applied to virtually any ESI–MS instruments with minor modifications.
Supplement
Detailed instructor notes, instructions for students, including postlab questions, and a program for the charge state deconvolution are available.
*  Contents Folder JCE2005p0099W containing:JCE2005p0099W.doc (Microsoft Word) and JCE2005p0099W.xls (Microsoft Excel)
*  Download
JCE2005p0099W.pdf

JCE2005p0099W.zip

JCE2005p0099W.sit
More Information
*  Citation
 Weinecke, Andrea; Ryzhov, Victor. J. Chem. Educ. 2005 82 99.
*  Keywords
 Analytical Chemistry; Instrumental Methods; Laboratory Instruction; Mass Spectrometry; Proteins / Peptides
*  History
 Created:
Last Updated:		 November 29, 2004
December 10, 2004
<< Previous Article Table of Contents Next Article >>
  Home > JCE Print > Journal of Chemical Education > Issues > 2005  > January  > Page 99


Subscriptions
 Subscription Info
 Order Forms

JCE HS CLIC

Our Secondary School editors work hard to distill all the JCE materials to produce a fraction of particular interest to high school teachers. We call it CLIC.
 JCE HS CLIC

Contributions Welcome
JCE welcomes your submission
 Contributors' Corner

Advertisers
In recent years we have worked hard to better match our advertisers with our readers. When shopping for chemistry education materials, visit our advertisers' WWW sites first.
 Recent Advertisers

Be An Ambassador
Take JCE along on your outreach missions. Copies of the Journal, guest access to JCE Online, our publications catalog, and more are available for your participants.
 Outreach with JCE

Comments to
jceonline@chem.wisc.edu		 Copyright © Division of Chemical Education, Inc., American Chemical Society.
All rights reserved.