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Movie Size - .6 MB Movie Duration - 20 Seconds File - CCA6/MOVIES/Labcurrent/spec20/01061300.MOV

Discussion

A reference solution is used to calibrate the scale. A reference solution is the same as the solution to be analyzed except that it must have zero concentration of the absorbing species.

It is a good idea to recheck the maximum absorbance after setting the zero absorbance to verify that the instrument is working properly.

Narrative

Wipe the cuvette containing the reference solution with a laboratory tissue. Align the mark as you insert the reference cuvette into the sample compartment and close the lid. Use the absorbance/transmittance control knob to set the display needle at zero absorbance.