Thin Layer Chromatography

CCA! Volume 6

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Thin Layer Chromatography

Techniques used in Thin Layer Chromatography are demonstrated.

Description

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Abbreviated TLC, thin layer chromatography is used largely as an analytical technique for determining the purity of materials and for preliminary identification purposes.

Preparation

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A developing chamber is prepared and the sample to be separated is spotted onto a TLC plate.

Developing the Chromatogram

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The spotted plate is placed in the chromatography tank and the chromatogram develops.

Identify and Interpret

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Methods to interpret the chromatogram and identify the separated spots are presented.