Preparation

CCA! Volume 6

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Preparation

A developing chamber is prepared and the sample to be separated is spotted onto a TLC plate.

Preparing the Development Chamber

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11 Slide(s)

A development chamber is prepared by adding solvent and filter paper to a container, and then allowing its atmosphere to become saturated with solvent vapor.

The Developing Tank

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The materials used to assemble a developing tank are shown.

Assemble the Developing Tank

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The developing tank is assembled.

Making a Spot Applicator

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18 Seconds

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A spot applicator is made from a capillary tube.

Draw Calibration Line

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A line is marked near the bottom of the chromatography plate above the level of solvent in the development chamber.

Preparing the Plates for Development (Part 1)

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26 Seconds

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Spot the sample on the plate with a drawn out capillary tube.

Preparing the Plates for Development (Part 2)

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To load more sample onto the plate, re-spot the solution over the first spot.

Mark the TLC Plate

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The TLC plate is marked with a pencil prior to placing the sample on the plate.

Spot the TLC Plate with Sample

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46 Seconds

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9 Slide(s)

The technique of spotting a TLC plate with sample is demonstrated.

Co-spotting Compounds

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51 Seconds

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The technique of co-spotting a thin layer chromatography plate is demonstrated.

Spots are too Low

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If the spots are placed too low on the chromatography plate, they will be washed off by the solvent.