Identify and Interpret

CCA! Volume 6

About CCA!

Methods to interpret the chromatogram and identify the separated spots are presented.

Spots may become visible when a TLC plate is viewed under ultra-violet light.

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.8 MB
25 Seconds

Spots are visualized and marked under an ultraviolet light.

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1.1 MB
38 Seconds

The plate is viewed under ultraviolet light and the separated spots that appear are marked with a pencil.

The plate is placed in an iodine chamber and a previously invisible spot appears.

The R_f value is explained.

Students might be asked to identify the problem in the pictures shown.

Two drawings of chromatograms are used for practice in calculating R_f values and identifying unknowns.