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  Home > JCE Print > Journal of Chemical Education > Issues > 2007  > December  >
In the Laboratory
Immobilized α-Galactosidase in the Biochemistry Laboratory
V. H. Mulimani and K. Dhananjay
Department of Biochemistry, Gulbarga University, Gulbarga-585106, Karnataka, India
Cover
December 2007
Vol. 84 No. 12
p. 1974

Abstract
This laboratory experiment was designed to demonstrate the application of immobilized galactosidase in food industry to hydrolyze raffinose family oligosaccharides in soymilk. This laboratory experiment was conducted for postgraduate students of biochemistry and developed for graduate and undergraduate students of biochemistry, biotechnology, chemical engineering, and food technology courses. The students were able to compare the hydrolysis of raffinose family oligosaccharides by free and immobilized-galactosidase. This experiment illustrates the basic concept of immobilization and its application.
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Directions for the students and notes for the instructors are available.
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Citation
Mulimani, V. H.; Dhananjay, K. J. Chem. Educ. 2007, 84, 1974.
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Keywords
Agricultural Chemistry; Biochemistry; Biotechnology; Carbohydrates; Enzymes; Food Science; Graduate Education / Research; Hands-On Learning / Manipulatives; Laboratory Instruction; Nutrition; Upper-Division Undergraduate
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History
Created:
Last Updated:
10/25/2007
10/31/2007
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Experiments, laboratory exercises, lecture demonstrations, and other descriptions of the use of chemicals, apparatus, instruments, computers, and computer interfaces are presented in the Journal of Chemical Education as illustrative of new or improved ideas or concepts in chemistry instruction and are directed at qualified teachers. Although every effort is made to assure and encourage safe practices and safe use of chemicals, the Journal of Chemical Education cannot assume responsibility for uses made of its published materials. Many chemicals are hazardous. Precautions for the safe use of hazardous chemicals and directions for their proper disposal are described in the Material Safety Data Sheets and on the labels. We strongly urge all those planning to use materials from our pages to make choices and to develop procedures for laboratory and classroom safety in accordance with local needs and situations.
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