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  Home > JCE Print > Journal of Chemical Education > Issues > 1996  > June  >
Laboratory Experiments
Research in Undergraduate Instruction: A Biotech Lab Project for Recombinant DNA Protein Expression in Bacteria
Mark Brockman and Alfred B. Ordman
Biochemistry Program, Beloit College, WI 53511

A. Malcolm Campbell
Dept. of Biology, Davidson College, NC 28036

Cover
June 1996
Vol. 73 No. 6
p. 542
Abstract
In the sophomore-level Molecular Biology and Biotechnology course at Beloit College, students learn basic methods in molecular biology in the context of pursuing a semester-long original research project. We are exploring how DNA sequence affects expression levels of proteins. A DNA fragment encoding all or part of the guanylate monokinase (gmk) sequence is cloned into pSP73 and expressed in E. coli. A monoclonal antibody is made to gmk. The expression level of gmk is determined by SDS gel elctrophoresis, a Western blot, and an ELISA assay. Over four years, an increase in enrollment in the course from 9 to 34 students, the 85% of majors pursuing advanced degrees, and course evaluations all support the conclusion that involving students in research during undergraduate courses encourages them to pursue careers in science.
More Information
*  Citation
 Brockman, Mark; Ordman, Alfred B.; Campbell, A. Malcom. J. Chem. Educ. 1996 73 542.
*  Keywords
*  History
 Created:
Last Updated:		 9/23/1999
5/22/2006
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